3D Printers are important elements for microfluidic research in current time. Our team use this technique for fabrication of various accessories to be used together with PDMS microfluidic devices such as fittings, connectors, holders, molds, manual valves, power free actuators, sensor cartridges, instrument prototypes, and more. 3D printing of these components both provide quick and affordable mass fabrication and more importantly ensure custom fabrication for the needs of special designs of the research. Although its increased popularity, the main limiting factor is the materials to be used with chips and it is needed to have better composite resins for biological and chemical studies.